2024Journal articleFree to read

Development of vitamin K analysis method using column switching high-performance liquid chromatography method and analysis results of various food items for vitamin K content

Rina Tanaka, Ryusuke Tanaka, Ryusuke Tanaka, Ryusuke Tanaka

Food Analytical Methods

Abstract

Abstract In this study, we developed a column-switching high-performance liquid chromatography (HPLC) method with fluorescence detection for the analysis of vitamin K. Column-switching is accomplished by changing the direction of flow using a switching valve with a set time program. Using this method, three vitamin K, phylloquinone (PK), menaquinone-4 (MK-4), and menaquinone-7 (MK-7), were separated and identified with high sensitivity, and impurities were eliminated. This method was used to determine the vitamin K content in meat, fish meat, snails, bivalves, sea urchins, seaweeds, vegetables, tea, soy products, milk products, and supplements. The results showed that chicken showed the highest content of MK-4 (15.35 ± 0.35 μg/100 g), matcha showed the highest content of PK (3069.66±80.10 μg/100 g), and dried natto showed the highest content of MK-7 (3997.57±79.42 μg/100 g). This method can also be used to analyze vitamin K in supplements and pharmaceuticals. The results of this study revealed that different manufacturers add different types of vitamin K to their commercial supplements and infant formulas. The developed method provides highly reproducible and quantitative results and allows for the rapid analysis of the three vitamin K types. Thus, the method developed in this study may aid the sequential analysis of vitamin K in different samples to assess food nutrients.

Abstract text comes from PubMed, OpenAlex or Europe PMC and belongs to the authors and publisher.

Study type

Chemical / analytical

Measuring what is in matcha, or how to detect it.

Assigned automatically from the title, abstract and subject terms.

Topics

Authenticity and analytical methods

Matcha is a main subject here (named in the keywords).

Keywords and subject terms

vitamin K vitamin K1 menaquinone-4 menaquinone-7 column switching switching valve fluorescence detection natto matcha infant formula

Cite this study

Rina Tanaka, Ryusuke Tanaka, Ryusuke Tanaka, Ryusuke Tanaka. (2024). Development of vitamin K analysis method using column switching high-performance liquid chromatography method and analysis results of various food items for vitamin K content. Food Analytical Methods. https://doi.org/10.1007/s12161-024-02643-4

DOI: 10.1007/s12161-024-02643-4 · Cited by 11 (OpenAlex)

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