2025PreprintFree to read
Emily Kipsura, Robert Koech, Simon Mwangi, Samson Machohi Kamunya, Victor Ngeno, Joel Koech, Christine C. Bii
bioRxiv (Cold Spring Harbor Laboratory)
ABSTRACT Diversification and value addition in tea has received considerable attention in recent past. This has been occasioned by increased tea volumes in the world market, especially the aerated product that has led to over flooding. Additionally, health benefits associated with diversified products such as green tea and extracts has elicited a lot of interests in both the industry and external players in finding out alternative ways of tea consumption. The aim of this study was to determine biochemical composition of the various tea extract products generated at different points of a multistage extraction process. The extraction process consisted of hot distilled water, methylene chloride and ethyl acetate steps. The products of purple, yellow, green, black orthodox teas, matcha and the semi aerated oolong were used as raw materials for the extracts. Total polyphenols content, individual catechins and caffeine content were assayed using UV-spectrophotometer and High Performance Liquid Chromatography-Photo diode array HPLC-PDA. Analysis of variance (ANOVA) was used for statistical purposes to detect significant differences between the extraction methods with the least significant difference (LSD) used to separate the means in the assayed tea samples. Results generated indicated that total polyphenols, individual catechins and caffeine content were significantly different (p≤0.05) in the assayed tea samples. Water extracts showed high total yield (82.2%), while methylene chloride recorded the least (2.63%). Ethyl acetate tea extracts showed high phenolic content that ranged from 11. 4% to78.1%. Ethyl acetate extract was rich in total catechins content (46.6% to 75.9%). Water extracts had significantly (p≤0.05) high amounts of total catechins (12.3% to 27.6%) compared…
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Study type
Chemical / analytical
Measuring what is in matcha, or how to detect it.
Assigned automatically from the title, abstract and subject terms.
Topics
Matcha is a main subject here (named in the keywords).
Emily Kipsura, Robert Koech, Simon Mwangi, Samson Machohi Kamunya, Victor Ngeno, Joel Koech, Christine C. Bii. (2025). BIOCHEMICAL COMPOSITION OF SPECIALTY TEA EXTRACTS FROM MULTISTAGE EXTRACTIONS USING SOLVENTS TREATMENTS. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2025.01.29.635473
DOI: 10.1101/2025.01.29.635473
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